centrifugation technique

Objective To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique .

目的探讨应用改良Percoll非连续密度梯度离心法分离纯化培养组织工程用肌卫星细胞。

A combination of continuous and interval Percoll density gradient centrifugation technique was used to isolate and purify epidermal LC from guinea-pig .

我们采用连续、间断Percoll密度梯度离心相结合的方法分离、纯化豚鼠表皮LC。

Objective : To probe into the application of 90 % percoll single-step gradient centrifugation technique in intrauterine insemination ( IUI ) .

目的探讨90%Percoll单层梯度离心法在宫腔内人工授精（IUI）中的应用价值。

[ Methods ] The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous Percoll density gradient centrifugation technique .

【方法】分别采用差速贴壁法和Percoll非连续密度梯度离心法进行新西兰兔肌卫星细胞的原代培养并进行纯化。

The differential centrifugation technique and sequential chemical extraction method were used to study the subcellular distribution and the chemical forms of Cu in the roots , leaves and stems of Commelina communis .

运用差速离心法和化学试剂逐步提取法分析了Cu在鸭跖草Commelinacommunis根、茎、叶的亚细胞分布及贮存形态。

[ Results ] Over 90 % satellite cells were harvested by incontinuous density Percoll gradient centrifugation technique , in contrast to which , only 30-40 % cells were harvested by different adhesion time .

【结果】两种方法均能分离培养获得肌卫星细胞，经测定差速贴壁法所得细胞纯度为30-40%，Percoll非连续密度梯度离心法所得细胞纯度在90%以上。

The mitochondria and microsome of testis cells were prepared by centrifugation technique to detect the levels of lipid peroxidation ( LPO ), total antioxide capacity ( T-AOC ), reactive oxygen species ( ROS ) in spectrophotometry .

制备睾丸组织匀浆，离心提取线粒体和微粒体，采用分光光度法检测睾丸细胞线粒体及微粒体中脂质过氧化物（LPO）、总抗氧化能力（T-AOC）、活性氧（ROS）水平的变化。

Applying rapid centrifugation smear technique to detect acid-fast bacilli in sputum of first visit pulmonary tuberculosis patients

采用快速离心沉淀法检测初诊肺结核患者痰内抗酸杆菌

Separation of these pure ice crystals , by centrifugation or some other technique , leaves a concentrated product .

用离心或其它的技术将这些纯冰晶分离出去。我们就会得到一种浓缩产品。

Objective : To explore the application of Percoll gradient centrifugation and Wang tube technique in sperm separation and their effects in artificial insemination with husband semen .

目的探讨精子经王氏管和密度梯度离心法处理后精子参数的变化，比较两者在宫内人工授精（IUI）的临床效果。